Three-Dimensional Fingerprint Spectroscopy Study on the Biopolymer System of Polyphenol Oxidase Binding with Cumalic Acid

The protection of Cumalic acid (CA), antioxidant, in the biochemical process nature has aroused great interest. Polyphenol oxidase (PPO), an enzyme, plays a vital function aging and browning plants, such as vegetables, fruits, mushrooms. interaction CA PPO reveals important information metabolism aging. Thus, molecular mechanism binding with polyphenol (PPO) was explored by combining spectroscopic methods modeling. A three-dimensional fingerprint CA-PPO complex built for first time to characterize biopolymer between PPO. Application indicated that effectively quenched intrinsic fluorescence enthalpy change (ΔH°) entropy (ΔS°) suggested predominantly stabilized hydrophobic interactions Building λ-UV-F made it possible demonstrate Subsequently, modeling demonstrated primarily bound hydrogen bonds located at amino residues Ala202, His38, His54, Ser206. computational simulations were consistent spectral experiments demonstrating confidence model determined interaction.